Protective effect of Naringenine on cisplatin-induced liver toxicity via suppressing inflammation and oxidative stress in mice

Abstract Background: Cisplatin (Cis) is a chemotherapeutic drug recognized for its hepatotoxic effects and its potential to trigger oxidative stress. This research aimed to evaluate the protective function of naringenin (NG) in mitigating Cis-induced liver toxicity by examining the inflammatory pathways and oxidative stress in mice. Methods: Adult male mice were given 50 mg/kg NG for two weeks, without Cis or with 10 mg/kg Cis on the 10th day. Histological analysis, biochemical indicators, and oxidative stress biomarkers were evaluated. The protein levels of Nrf2 and NF-κB were also measured to assess inflammation within the liver tissue. Results: The current research revealed a notable rise in the levels of Nrf2, AST, ALT, ALP, and MDA, alongside a marked reduction in the levels of NF-κB, CAT, GSH, and SOD in the Cis group. Pretreatment with NG resulted in a decrease in biochemical markers, histological alterations, and oxidative stress. NG was found to lower Nrf2 protein levels while elevating the levels of NF-κB protein in the Cis-intoxicated mice. Conclusion: The findings indicated that NG exerts a protective influence against Cis-induced hepatotoxicity by reducing histological alterations, oxidative stress, and inflammation.